Description

HOT FIREPol^® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water).

Generated PCR products are compatible with TA cloning procedures

Properties

Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Ready to load: no
Fidelity: 1 x Taq
Cloning Type: T/A cloning
Multiplexing: up to 18 targets (depending on amplicon length)
Amplicon Size: up to 5 kb

Mix Components

• HOT FIREPol^® DNA polymerase
• 5x Multiplex Buffer with 10 mM MgCl₂ (1x PCR solution – 2 mM MgCl₂)
• dNTPs 
• BSA
• Compound that increases sample density (loading dyes not included