Description

FIREScript^® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C.

FIREScript^® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

Applications

• First-strand cDNA synthesis
• RT-PCR
• RT-qPCR

Reagents

• FIREScript^® Reverse Transcriptase (200 U/μl)
• RiboGrip^® RNase inhibitor (220 U/μl)
• 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl₂, 100 mM DTT)
• Oligo (dT) Primer (100 μM)
• Random Primers (100 μM)
• dNTP MIX (20 mM of each)
• Water, nuclease free