Brand: Solis BioDyen
Size: 50 rxn | 50 x 20 μl rxn

Description
SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.
It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube

Applications
Detection and quantification of RNA targets with qPCR
DNA/LNA hydrolysis probe based assay
Suitable for singleplex or duplex reactions

Properties
Sample type: RNA
Product format: Reagent kit
No of tubes: three tubes
Detection type: Probe-based
Reference dye: reference dye based on ROX
Compatible real-time instruments: The Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

Reagents
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), internal reference based on ROX dye
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor
Water, nuclease free